Isogeneic bone marrow mesenchymal stem cells transfusion combined donor spleen tissue transplantation induces allograft tolerance in liver transplant rats
10.3969/j.issn.1673-8225.2010.18.004
- VernacularTitle:输注同基因骨髓间充质干细胞联合脾组织移植诱导肝移植大鼠的免疫耐受
- Author:
Qian WANG
;
Li LI
;
Xiaoyan LI
;
Gang CHEN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(18):3243-3246
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Studies have shown that isogeneic splenocyte transplantation can induce immune tolerance and prolong liver graft survival under the effect of immunosuppressant. It is reported that bone marrow mesenchymal stem cells (BMSC) transplantation can also prolong the liver graft survival.OBJECTIVE: To observe the antirejection effect on liver grafts by simultaneous transplantation of isogeneic BMSC and spleen tissue in liver transplant rats.METHODS: Recipient Lewis rats were randomly divided into 4 groups: in acute rejection group, DA rats as the donor and Lewis rats as the recipients for liver transplantation; in cyclosporine A (CsA) group: recipients received CsA after liver transplantation. In the BMSC group, recipient Lewis rats received simultaneous BMSC transfusion and liver transplantation. In the spleen tissue group, the recipient Lewis rats received DA rat liver, spleen tissue and isogeneic BMSC transplantation. The serum cytokine levels were determined, and liver pathological changes and survival were observed postoperatively. The level of chimerism in the spleen of Lewis rats were also observed.RESULTS AND CONCLUSION: The blood levels of alanine aminotransferase, aspartate aminotransferase and total bilirubin interleukin-2 (IL-2) and interferon gamma of spleen tissue group were significantly reduced compared with the other groups (P< 0.05), but IL-6 and IL-10 were increased (P< 0.05), and donor positive cells were increased at 30 days (P< 0.05). Liver pathology revealed a mild acute rejection in liver graft of CsA and BMSC groups, severe acute rejection in acute rejection group, but no rejection in spleen tissue transplantation group. Results indicate that isogeneic BMSCs transfusion combined donor spleen tissue transplantation can induce allograft tolerance in liver transplant rats.
