Construction of engineered myocardial tissues with polylactic-co-glycolic acid polymer and cerdiomyocyte-like cells in vitro
10.3969/j.issn.1673-8225.2010.16.007
- VernacularTitle:聚乳酸-聚乙醇酸共聚物复合心肌样细胞体外构建工程化心肌组织
- Author:
Yujie XING
;
Anlin Lü
;
Li WANG
;
Xuebo YAN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(16):2875-2878
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Previous research has demonstrated that bone marrow mesenchymal stem calls (BMSCs) differentiate into varying mesoderm-derived mesenchymal cells, such as myocardial cells.OBJECTIVE: To investigate the feasibility of construction of engineered myocardial tissues with polylactic-co-glycolic acid (PLGA) olymer and cardiomyocyte-like cells derived from BMSCs in vitro.METHODS: BMSCs were isolated from bone marrow of SD rats by density gradient cantrifugation. The third passage cells were induced with the culture medium including 5-aza for 4 weeks. After successful induction, the cells were trypsinized and suspended, then the cell suspension was added to the polylactic-co-glycolic acid square slowly and cultured in the incubator for 14 days. The morphological changes were observed before and after induction under phase contrast microscope. The cardiomyocyte-like cells were identified by immunofluorescanca staining. The naked eyes were used to observe the morphology of engineered myocardial tissues. The ultrastructures of the engineered myocardial tissues were viewed with a transmission electron microscope.RESULTS AND CONCLUSION: BMSCs of primary culture formed cell colonies at 14 days. The passaged cells were larger than those of primary culture. After induction by 5-aza, the cells presented long spindle and aligned in parallel. The expression of specific proteins of cardiac troponin Ⅰ (cTnl) in induced BMSCs was positive. Transmission electron microscopy showed that the engineered myocardial tissues had myofilaments, Z line-like substances. With PLGA and cardiomyocyte-like cells derived from BMSCs, the engineered myocardial tissues which resemble native cardiac tissues can be successfully constructed in vitro.
