Molecular biological mechanism of osteolysis induced by titanium wear particles of artificial joint
10.3969/j.issn.1673-8225.2010.26.044
- VernacularTitle:人工关节磨损钛微粒诱导破骨的分子生物学机制
- Author:
Gang WANG
;
Qing CAI
;
Shiqing LIU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(26):4929-4932
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Under wear particles stimulation, mononuclear macrophages, fibroblasts, and osteoblasts can produce a large amount of inflammatory factors, leading to periprosthetic osteolysis. But the precise mechanisms remain unclear. OBJECTIVE: To analyze the molecular biological mechanism underlying osteolysis induced by titanium wear particles . RESULTS AND CONCLUSION: Cleaned titanium particles stimulation induced an unbalanced ratio of RANK mRNA to OPG mRNA. Over-expressed RANK bound to RANK ligand and promoted osteolysis. No RANK mRNA expression was detected in the LPS group, but OPG mRNA expression was transiently increased at 4 hours. NF-κB/inflammatory cytokine, rather than RANK/OPG, is the main signal pathway for LPS to induce osteolysis. After LPS binding to titanium particles, these two signal mechanisms, RANK/OPG and NF-κB/inflammatory cytokine, have synergistic effects during artificial joint loosening.
