Effect of GnRH Ⅱ and GnRH Ⅰ on secretion of VEGF by eutopic and ectopic endometrial stromal cells of endometriosis patients
10.3969/j.issn.1672-7347.2010.05.002
- VernacularTitle:GnRH Ⅱ与GnRH Ⅰ对子宫内膜异位症患者间质细胞分泌VEGF作用的比较
- Author:
Fengying HUANG
;
Qiuhong LIU
;
Huanping WANG
;
Ying ZOU
- Publication Type:Journal Article
- Keywords:
endometriosis;
gonadotropin-releasing hormone Ⅱ(GnRH Ⅱ);
GnRH Ⅰa(goserelin);
vascular endothelial growth factor (VEGF);
endometrial stromal cell
- From:
Journal of Central South University(Medical Sciences)
2010;35(5):409-418
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of GnRH Ⅰ and GnRH Ⅱ on the secretion of VEGF by eutopic and ectopic endometrial stromal cells cultured in vitro, and to provide theoretical basis for exploring new treatments for endometriosis (EMs).Methods Eutopic and ectopic endometrium stromal cells cultured in vitro were treated with different concentrations of GnRH Ⅱ and a GnRH I(goserelin), and a control group was not treated by GnRH Ⅱ and GnRH Ⅰ. Enzyme linked immunosorbent assay (ELISA) was used to measure the content of vascular endothelial growth factor (VEGF) protein in the medium of the above 2 groups.Results (1)There was no difference in the VEGF protein secreted by eutopic and ectopic stromal cells in the medium after being cultured in vitro for 48 h (P＞0.05).(2)10-10, 10-8, and 10-6mol/L GnRH Ⅱ dose-dependently reduced VEGF protein secreted by endometrial stromal cells (P＜0.05),and the inhibition effect was stronger than that of GnRH Ⅰ (P＜0.05).(3)The inhibition effect of GnRH Ⅱ on VEGF in ectopic stromal cells was stronger than that of eutopic stromal cells (P＜0.05).Conclusion (1)Ectopic stromal cells cultured in vitro can secrete VEGF,which has no difference from the eutopic stromal cells, and which may play an important role in the formation and development of EMs.(2)GnRH Ⅱ can dose-dependently reduce VEGF protein secreted by ectopic and eutopic endometrial stromal cells cultured in vitro,and the inhibition effect is stronger than that of GnRH Ⅰ, providing theoretical basis for exploring new treatments for EMs.
