Influence of intense pulsed light on the secretion of TGF-β1 in cultured human fibroblasts and intervention of JNK inhibitor
10.3969/j.issn.1672-7347.2010.05.003
- VernacularTitle:强脉冲光对成纤维细胞分泌TGF-β1的影响及JNK抑制剂的干预作用
- Author:
Jianyun LU
;
Xiaofeng YU
;
Jian KANG
;
Zehui OUYANG
;
Xuerui GENG
;
Yaping XIANG
;
Jinhua HUANG
- Publication Type:Journal Article
- Keywords:
intense pulsed light;
fibroblasts;
JNK;
transforming growth factor β1
- From:
Journal of Central South University(Medical Sciences)
2010;35(5):419-423
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the influence of intense pulsed light (IPL) on the secretion of TGF-β1 in cultured human fibroblasts and the intervention of JNK inhibitor.Methods The callan foreskin fibroblasts were cultured and divided into 2 groups. In the IPL treatment group, cells were irradiated with IPL with fluences of0 (negative control), 10, 18, 27, 36, and 36 J/cm2 × 2 (irradiated with IPL with fluences of 36 J/cm2 twice). In the IPL + inhibitor group, cells were irradiated with IPL with fluences of 36 J/cm2 after incubation with the inhibitor SP600125 for 2 h. TGF-β1 in the culture supernatant was evaluated 48 h after the irradiation using enzyme-linked immunosorbent assay. Results Compared with the negative control, TGF-β1 in the culture supernatant decreased at the IPL irradiation of 10, 18, 27, and 36 J / cm2, whereas TGF-β1 increased at the IPL irradiation of 36 J/cm2× 2. In the IPL + inhibitor group, the concentration of TGF-β1 in the culture supernatant decreased compared with the controls (P<0.05). Conclusion IPL can suppress the secretion of TGF-β1 at the lower fluence and promote the secretion at a higher fluence. JNK inhibitor may play an inhibitive role when IPL regulates the TGF-β1 secretion in cultured human fibroblasts. IPL may stimulate TGF-β1 secretion of the fibroblast cells in human skin via JNK signal pathway.
