Endogenous stress contributes to cardiac injury after heart transplantation in rats
10.3969/j.issn.1672-7347.2010.05.004
- VernacularTitle:内源性应激损伤对移植后大鼠心脏病变的影响
- Author:
Wei ZHUANG
;
Li LI
;
Haihe JIANG
;
Weixing ZHANG
;
Shengxi CHEN
- Publication Type:Journal Article
- Keywords:
rat;
heart transplantation;
cardiac allograft vasculopathy (CAV);
stress injury;
proteome
- From:
Journal of Central South University(Medical Sciences)
2010;35(5):424-430
- CountryChina
- Language:Chinese
-
Abstract:
Objective Stress effect plays an important role in the development of some myocardial diseases. We hypothesized it was important nosogenesis to myocardial damage and cardiac allograft vasculopathy. Methods The transplanted hearts from Lewis to Wister rats served as allografts and from Lewis to Lewis rats as isografts based Ono's model. The differential proteins in the transplanted hearts were separated by comparative proteome, and then identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and searched by Matrix Science software system.Results All transplanted hearts were characterized by lumen loss [(total vessel area-luminal area)/total vessel area] in the coronary artery 2 weeks after the operation [(2.07%±0.93%) vs. (27.58%±11.14%), P＜0.01], but more predominant after 8 weeks [(2.34%±1.06%) vs. (72.29%±20.57%), P＜0.01]. All samples of the left ventricle were analyzed by proteomic techniques and 37 distinct proteins involving their respective isoforms and subunits were identified. Nine proteins were correlated to endoplasmic reticulum stress effect and myocardial damage, and 2 proteins were verified by Western blot.Conclusion Stress plays an important role in cardiac allograft damage and the development of rat cardiac allograft vasculopathy.
