Expression and Immunological Analysis of Capsid Protein Precursor of Swine Vesicular Disease Virus HK/70
10.1007/s12250-010-3100-x
- Author:
Hong TIAN
;
Jingyan WU
;
Youjun SHANG
;
Shuanghui YING
;
Haixue ZHENG
;
Xiangtao LIU
- Publication Type:Journal Article
- Keywords:
Swine vesicular disease virus;
Capsid protein precursor gene (vpl);
Gene expression;
Immune response
- From:
Virologica Sinica
2010;25(3):206-212
- CountryChina
- Language:Chinese
-
Abstract:
VP1, a capsid protein of swine vesicular disease virus, was cloned from the SVDV HK/70 strain and inserted into retroviral vector pBABE puro, and expressed in PK15 cells by an retroviral expression system. The ability of the VP1 protein to induce an immune response was then evaluated in guinea pigs. Western blot and ELISA results indicated that the VP1 protein can be recognized by SVDV positive serum, Furthermore,anti-SVDV specific antibodies and lymphocyte proliferation were elicited and increased by VP1 protein after vaccination. These results encourage further work towards the development of a vaccine against SVDV infection.
