Complete Genomic Sequence of Transmissible Gastroenteritis Virus TS and 3'End Sequence Characterization Following Cell Culture
10.1007/s12250-010-3108-2
- Author:
Jianqiang LI
;
Jie CHENG
;
Xi LAN
;
Xuerui LI
;
Wei LI
;
Xiangping YIN
;
Baoyu LI
;
Bin YANG
;
Zhiyong LI
;
Yun ZHANG
;
Jixing LIU
- Publication Type:Journal Article
- Keywords:
Cloning;
Complete genome;
Gastroenteritis virus (TGEV);
Cell culture
- From:
Virologica Sinica
2010;25(3):213-224
- CountryChina
- Language:Chinese
-
Abstract:
The complete genome sequence of transmissible Gastroenteritis virus (TGEV) strain TS, previously isolated from Gansu province, was cloned and compared with published sequence data from other TGEV strains.Phylogenetic tree analysis based on the amino acid and nucleotide sequences of the S gene showed that the TGEV strains were divided into 3 clusters. TGEV TS showed a close evolutionary relationship to the American Miller cluster but had a 5' non-translated region (NTR) sequence closely related to the American Purdue cluster.Continued culture in different cell types indicated that TGEV TS virulence could be attenuated alter fifty passages in Porcine kidney (PK-15) cells, and that the Porcine kidney cell line IB-RS-2 (IBRS) was not suitable for culture of the TGEV strain TS.
