Expression of steroidogenic acute regulatory protein following with age and serum lipids levels in apolipoprotein E-deficient mice
- VernacularTitle:不同月龄和血脂水平ApoE基因敲除小鼠体内类固醇激素合成急性调节蛋白的表达
- Author:
Yanxia NING
;
Yifeng JIANG
;
Chen XU
;
Fengdi ZHAO
;
Lianhua YIN
- Publication Type:Journal Article
- Keywords:
steroidogenic acute regulatory protein;
cholesterol;
apolipoprotein E-deficient mice;
C57BL/6J mice;
oxysterols
- From:
Fudan University Journal of Medical Sciences
2010;37(4):422-429
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect steroidogenic acute regulatory protein (StAR) expression in apolipoprotein E-deficient mice at different ages and serum lipid levels. Methods Nighty-six C57BL/6J and apoE-/- mice were enrolled, which were divided into 16 groups with 6 mice per group according to age (1 day, 1, 3, 5 months), sex and genotype (C57BL/6J and apoE-/-). The serum lipid levels in C57BL/6J and apoE-/- mice were detected by commercial kits. StAR mRNA and protein expressions in liver were detected by Real-time PCR and Western blot respectively. Results ApoE-/- mice had higher LDL-cholesterol and lower HDL-cholesterol compared with C57BL/6J mice of the same age and sex. StAR mRNA and protein expressions were decreased following with aging in C57BL/6J mice. However, in apoE-/- mice with higher lipid levels, StAR mRNA and protein expressions were changed with the lipid levels other than ages. StAR mRNA and protein increased in the early stage, and then decreased with the increasement of lipids levels. Conclusions StAR could affect lipids levels and may be an effective regulator for atherosclerosis and other cardiovascular diseases.
