HLA-peptide tetramers and adoptive immunotherapy in prevention of cytomegalovirus disease
10.3969/j.issn.1673-8225.2010.31.044
- VernacularTitle:HLA-肽四聚体和过继性免疫疗法与巨细胞病毒疾病
- Author:
Guangping RUAN
;
Xiang YAO
;
Xinghua PAN
;
Rongqing PANG
;
Yongli DENG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(31):5878-5882
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Antiviral drugs can reduce the incidence of early-onset cytomegalovirus(CMV)disease,but are associated with strong toxicity and the development of late-onset CMV disease.In order to prevent CMV disease better,cytotoxic T lymphocytes(CTL)may play a critical role in controlling CMV reactivation.Fluorescent HLA-peptide tetramers are used to monitor the recovery of CMV CTL in recipients of allogeneic transplants.OBJECTIVE: To explore the effect of HLA-peptide tetramers and adoptive immunotherapy in treating CMV disease.METHODS: A computer-based online search of Pubmed and Wanfang databases was performed for articles related to CTL detection,application of antiviral drugs and HLA-peptide tetramers,and adoptive immunotherapy with key words"HLA-peptide tetramers,cytomegalovirus,specific CTL,adoptive immunotherapy"in English and Chinese.Repetitive articles were excluded and 29 articles were included.RESULTS AND CONCLUSION: Adoptive immunotherapy with CMVs cytotoxic T cells as preemptive therapy is a very elegant strategy; however,generation of these cells is costly and time-consuming,and therefore the therapy is not available at every transplantation center.Magnetic selection of CMVs CD8+T cells from peripheral blood of CMV-seropositive donors by using HLA-peptide tetramers may be very hopeful,which simplifies adoptive immunotherapy.
