Effects of angelica polysaccharides on the proliferation of mouse skeletal muscle satellite cells in hematopoietic microenvironments in vitro
10.3969/j.issn.1673-8225.2010.40.039
- VernacularTitle:当归多糖对体外造血微环境中肌卫星细胞增殖的影响
- Author:
Tao WANG
;
Li FENG
;
Xiaoling WANG
;
Hailin SONG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(40):7580-7582
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:It is hopeful that skeletal muscle satellite cells(SMSCs)can be served as seed cells for hematopoietic reconstitution.Angelica polysaccharides(APS)can not only promote hematopoietic stem cells and hematopoietic progenitor cells proliferation and differentiation,but also change the growth characteristics of SMSCs.OBJECTIVE:To investigate the effects of APS on the proliferation of mouse SMSCs in different culture environments.METHODS:SMSCs were procured by a modified method from new born mouse.The α-actin protein of the SMSCs was examined by immunohistochemistry at 5 days after culture.SMSCs were cultured and synchronized for 24 hours in the 96-well plate.After that,SMSCs were assigned into the blank control group,marrow stroma cell supernatant group,APS DMEM/F12 groups(contained 50,100,200,300,400 mg/L APS)and the marrow stroma cell conditioned medium(disposed by 50,100,200,300,400 mg/L APS in DMEM/F12).The proliferation of SMSCs was determined by MTT.RESULTS AND CONCLUSION:The α-actin was positive in the cultured SMSCs.MTT results demonstrated that,SMSCs showed a proliferative property in the marrow stroma cell conditioned medium groups.Additionally,the marrow stroma cell conditioned medium can effectively alter growth characteristics of SMSCs in a dose-dependent manner.
