Maintenance of undifferentiated state of human embryonic stem cells in chemical defined medium at high clone density without exogenous cell factors

VernacularTitle:无饲养细胞无外源因子条件下高克隆密度维持人类胚胎干细胞自身未分化状态
Author: Shuwei LUO ; Ge LIN ; Zhen SUN ; Pingyuan XIE ; Tiancheng LIU ; Guangxiu LU
Publication Type:Journal Article
Keywords: human embryonic stem cells; feeder cells; autocrine
From: Journal of Central South University(Medical Sciences) 2010;35(11):1123-1128
CountryChina
Language:Chinese
Abstract: Objective To establish human embryonic stem cells (hESCs) feeder-independent and cell factor-free culture system. Methods Effect of high and low clone densities of hESCs culture was compared and impact of the clone densities to hESCs culture media was analyzed. Results HESCs could maintain their undifferentiated states at high clone density (34 clones/cm2) without cell factors. At the same time,the bone morphology protein (BMP)-like induction of N2 and B27 supplements (NB) medium could be modulated by the clone density,and high level of BMP-like induction was accompanied by high clone density. Conclusion High clone density of hESCs can change the environments by themselves to maintain the undifferentiated states,which provides a new clue to explore the mechanism of undifferentiated states of hESCs and simplify the culture medium.