Comparison of differential proteomics between neural stem cells and motor neurons in embryonic spinal cord in rats

VernacularTitle:大鼠脊髓源神经干细胞与运动神经元的差异蛋白质组学
Author: Chunfang WANG ; Pengfei LI ; Hongen WEI ; Fei WANG ; Shufeng HAN ; Shudang WANG ; Yan DOU ; Jiahui ZHAO
Publication Type:Journal Article
Keywords: Neural stem cell; Motor neuron; Spinal cord; Proteomics; Rat
From: Acta Anatomica Sinica 2010;41(6):779-784
CountryChina
Language:Chinese
Abstract: Objective To analysis and compare the proteomic differences between neural stem cells and motor neurons in embryonic spinal cord in rat and discover the key different proteins. Methods Separating the protein of cells by the 2-D fluorescence difference gel electrophoresis, and to analyse the differences of protein expression with DeCyder software, and to identify with high performance liquid chromatography-electrospray tandem mass spectrometry. Results About 1 300 protein spots from the cells were gained after gel analysis. Eighty-seven protein spots were selected for mass spectrometry analysis. Fourty-four differently expressed proteins (24 in neural stem cells and 20 in motor neurons) were identified by mass spectrometry analysis.Conclusion Differently expressed proteins between neural stem cells and motor neurons were identified and it is helpful to find the new targets in neural stem cells differentiation into motor neurons.