Effect of docetaxel on expression of eIF3a in human lung cancer A549 cell line

VernacularTitle:多西他赛对肺癌A549细胞中eIF3a表达的影响
Author: Zhicheng GONG ; Xiaojing XU ; Ruiying HOU ; Yue GUO ; Feifeng SHENG ; Dongsheng OUYANG ; Honghao ZHOU ; Zhaoqian LIU
Publication Type:Journal Article
Keywords: docetaxel; eukaryotic initial factor 3a (eIF3a); α-tubulin; lung cancer
From: Journal of Central South University(Medical Sciences) 2010;35(8):771-776
CountryChina
Language:Chinese
Abstract: Objective To explore the dose-dependent and time-dependent effect of docetaxel on the expression of mammalian eukaryotic initiation factor 3 subunit A (eIF3a) in lung cancer cell line. Methods The human lung cancer cell line A549 was treated with gradient concentrations of docetaxel for different time. Real-time PCR and Western blot were used to detect mRNA and protein expression levels of eIF3a and α-tubulin, respectively. Results Docetaxel did not affect α-tubulin expression at either mRNA level or protein level. When A549 cells were treated with high concentration of docetaxel (30 μg/L), the expression level of eIF3a mRNA tended to increase in a time-dependent manner. Protein expression level of α-tubulin was not associated with eIF3a expression significantly in cells treated by docetaxel.Conclusion Docetaxel could slightly increase the expression of eIF3a mRNA, and eIF3a does not regulate the expression of α-tubulin in A549 cells treated by docetaxel.