Proteomics of apoptosis of multiple myeloma cells induced by proteasome inhibitor PS-341
10.3969/j.issn.1672-7347.2010.08.003
- VernacularTitle:蛋白酶体抑制剂PS-341诱导骨髓瘤细胞凋亡的蛋白质组学研究
- Author:
Haitao JIA
;
Feng GE
;
Xinpeng LU
;
Huilan ZENG
;
Liping LI
;
Zhipeng CHEN
;
Chunhua LU
- Publication Type:Journal Article
- Keywords:
PS-341;
multiple myeloma;
2-dimensional gel electrophoresis;
mass spectrometry;
drug target
- From:
Journal of Central South University(Medical Sciences)
2010;35(8):784-791
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the proteome difference between multiple myeloma cell line U266 cells treated and untreated with PS-341, to investigate the potential drug targets, and to provide theoretical evidence for clinical therapy of multiple myeloma. Methods Two-dimensional gel electrophoresis (2-DE) was performed to separate proteins from treated and untreated U266 cells with proteasome inhibitor PS-341. ImageMaster 2D Platinum software was used to analyze 2-DE image, and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) was used to identify the differentially expressed proteins. The expression levels of differential protein BAG-2 in the 2 groups of U266 cells lines were detected by Western blot. Results The 2-DE reference pattern of treated and untreated U266 cells with PS-341 was established. A total of 31 differential proteins were identified by MALDI-TOF-MS, 27 of which were down-regulated after PS-341 treatment. The differential expression level of BAG-2 in the 2 groups of U266 cells was confirmed by Western blot. Conclusion Some down-regulated proteins may be the potential drug targets of proteasome inhibitor PS-341.
