Cxcl16 Interact With SARS-CoV N Protein In and Out Cell
10.1007/s12250-010-3129-x
- Author:
Yuanpeng ZHANG
;
Rongwu ZHANG
;
Weishan CHANG
;
Yanwang YAN
- Publication Type:Journal Article
- Keywords:
SARS-CoVN;
CXCL16;
Eukaryotic expression;
Confocal laser scanning
- From:
Virologica Sinica
2010;25(5):369-374
- CountryChina
- Language:Chinese
-
Abstract:
Our study investigated the host cell protein which can interact with SARS-CoV N protein, and explored the functional connections. The eukaryotic expression vectors pEGFP-N1/SARS-CoVN and pdsRed2-N1/ CXCL16 were constructed and used to co-transfect HEK293FT cells by the calcium phosphate method. The HIS-tagged fusion protein SARS-CoVN-GFP was then built and purified for the binding assay in vitro. The co-localization of SARS-CoVN and CXCL16 in the cytoplasm of HEK293FT cells was also shown using confocal laser scanning microscopy. It is suggested that their interaction might be through direct combination. Under a fluorescence microscope, it was observed that the purified fusion protein SARS-CoVN-GFP was attached to the cell membrane of CXCL16-transfected cells, indicating that SARS-CoVN and CXCL16 can be mutually combined.
