Changes of PKAC-β, c-Fos and BDNF in cerebral cortex after intracerebral hemorrhage in rats treated with WIN55-212-2
10.3969/j.issn.1000-4718.2010.09.014
- VernacularTitle:大麻素WIN55-212-2对脑出血大鼠皮质内PKAC-β、c-Fos及BDNF表达量的影响
- Author:
Li ZHU
;
Zhi DONG
;
Guodong ZHANG
- Publication Type:Journal Article
- Keywords:
WIN55-212-2;
Cerebral hemorrhage;
Protein kinase A;
c-Fos;
Brain-derived neurotrophic factor
- From:
Chinese Journal of Pathophysiology
2010;26(9):1728-1733
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of cannabinoid receptor (CB1R) agonist WIN55-212-2 on the expression of brain-derived neurotrophic factor (BDNF), c-Fos and protein kinase A beta-catalytic subunit (PKAC-β) in cerebrum cortex after intracerebral hemorrhage (ICH) in rats. METHODS: The intracerebral hemorrhage model of rat was made by the injection of collagenase Ⅶ, and WIN55-212-2 was intraperitoneally (ip) injected 30 min later. The rats were killed for sampling the brain tissues as specimens 24 h after ICH. The methods of immunohistochemical analysis and Western blotting were adopted to detect the expression of PKAC-β and BDNF. The mRNA expression of PKAC-β, c-Fos and BDNF was determined by RT-PCR. RESULTS: WIN55-212-2 obviously improved some nervous deficit symptoms and increased the expression of BDNF at mRNA and protein levels with upregulating the mRNA expression of c-Fos and downregulating the expression of PKA at mRNA and protein levels in the ipsilateral cerebral cortex. The proteins of PKAC-β, c-Fos and BDNF were expressed on the membrane or nucleus of the neuron or in the cytoplasm of glial cells, respectively. CONCLUSION: The expression of BDNF is induced not only by upregulation of c-Fos, but also by downregulation of PKA in WIN55-212-2 treated rats.
