Brain-derived neurotrophic factor, ciliary neurotrophic factor and their combination for in vitro differentiation of human umbilical blood-derived mesenchymal stem cells into nerve-like cells
10.3969/j.issn.1673-8225.2010.19.040
- VernacularTitle:脑源性神经营养因子和睫状神经营养因子单独或联合体外诱导人脐带血来源的间充质干细胞向神经样细胞分化
- Author:
Wei HUANG
;
Zongning MIAO
;
Lei CHEN
;
Xueguang ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(19):3606-3610
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Umbilical cord blood mesenchymal stem cells (UCBMSCs) have been shown to differentiate into nerve-like cells under the condition of in vitro culture. Brain-derived neurotrophic factors (BDNF) combined with ciliary neurotrophic factors (CNTF) at certain concentration can in vitro induce the differentiation of UCBMSCs into high proportion of neuronal-like cells. OBJECTIVE: To investigate the feasibility of BDNF, CNTF and their combination for in vitrodifferentiation of UCBMSCs into nerve-like cells.METHODS: UCBMSCs of passage 5 were induced to differentiate into nerve-like cells using 5,10, 20 ug/L BDNF, 5,10, 20 ug/L CNTF or their combination. A blank control group was set, with no any interventions. Cell morphology was observed under inverted phase contrast microscope. At 1, 3, and 6 days of experiment, immunocytochemical staining for neuron specific enolase and glial fibrillary acidic protein was performed. The proportion of differentiated neuronal-like cells and glial cell-like cells was calculated.RESULTS ANDCONCLUSION:After induced differentiation into nerve-like cells, human UCBMSCs exhibited changeable morphology with contracted cell body, enhanced refraction of nucleus, and dendrite- and axon-like structure. Compared with blank control group, BDNF and CNTF couldsignificantly enhance the differentiation proportion of UCBMSCs into nerve-like cells. 20 ug/LBDNF combined with 20 ug/L CNTF yielded highest differentiation proportion of human UCBMSCs into nerve-like cells. These findings indicate that human UCBMSCs can differentiate into nerve-like cells after in vitro induction of BDNF, CNTF or their combination.
