Nano-hydroxyapatite/collagen composite co-cultured with bone marrow-derived mesenchymal stem cells for repair of bone defects
10.3969/j.issn.1673-8225.2010.42.044
- VernacularTitle:纳米晶羟基磷灰石胶原复合骨髓间充质干细胞修复骨缺损
- Author:
Jidong ZHAO
;
Hanguang QIAN
;
Zongning MIAO
;
Jianzhong ZHU
;
Wei PENG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(42):7971-7975
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: There have been no effective means for clinical treatment of large regions of bone defects.Nano-hydroxyapatite/collagen(nHAC)composite would provide a new pathway for repair of bone defects owing to its similar structure to natural skeleton and better biocompatibility.OBJECTIVE: To investigate the role of nHAC composite co-cultured with bone marrow-derived mesenchymal stem cells(BMSCs)in repair of bone defects.METHODS: Following isolation and culture,human BMSCs were co-cultured with nHAC composite.Gross observation,histological analysis,and electron microscope observation were performed to analyze osteogenesis for repair of bone defects in the clinic.RESULTS AND CONCLUSION: Human nHAC could greatly proliferate in vitro.X-ray photography revealed that bone defects well healed after implantation of nHAC/BMSCs composite.These findings indicate that BMSCs exhibit osteogenic potential and nHAC is a satisfactory scaffold material for construction of tissue-engineered bone.
