Effect of gadolinium chloride hexahydrate on rat hepatocellular mitochondria du ring liver cold ischemia storage
- VernacularTitle:氯化钆对冷保存鼠肝细胞线粒体的影响
- Author:
Yong ZHOU
;
Wenhai ZHANG
;
Jinsheng WANG
- Publication Type:Journal Article
- From:
Chinese Journal of General Surgery
2001;16(1):21-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of gadolinium chl oride hexahydrate (GDCL3) on the structure and lipid peroxide (LPO) of rat hep atocellular mitochondria during liver cold ischemia storage. Methods Forty Wistar rats were randomized into 8 gr oups ( 5 in each group). Group 1～4 served as control. The other 4 groups were p retreated with GDCL3 (7mg/kg). The ultrastructures of hepatocellular mitocho ndria were observed by electron microscope and LPO of hepatocellular mitochondri a were investigated. Results LPO o f hepatocellular mitochondria in group 1h, 2h, 3h pretreated with GDCL3 for 1、 2、 3 h were 1.0±0.3、1.7±0.2、2.0±1.0, LPO was suppressed sign ificantly compared with control group (2.2±1.0、2.8±1.0、3.5±1.0、 P<0.05). With time prclonging of liver storage, the damage of hepatocellular mitochondria ultrastructure became more severe and LPO of hepatocellular mitoch ondria increased significantly. Pretreated with GDCL3, the damage of hepatocel lular mitochondria ultrastructures were lessened and the elevation of LPO was s uppressed significantly (1 hour、 2 hours、3 hours，P<0.05). Conclusion Mitochondria damage is one of the sensitive characters of liver cold ischemia injury, GDCL3 inhibits rat hepatoc ellular injury during liver cold ischemia storage.
