Metabonomic analysis of rat urine 1H magnetic resonance spectra based on different normalization methods
10.3969/j.issn.1672-7347.2010.12.002
- VernacularTitle:标准化方法对大鼠尿液1H磁共振谱代谢物分析结果的影响
- Author:
Mamtimin BATUR
;
Hasim AYSHAMGUL
;
Chunli CHEN
;
Upur HALMURAT
- Publication Type:Journal Article
- Keywords:
metabonomics;
magnetic resonance;
normalization
- From:
Journal of Central South University(Medical Sciences)
2010;35(12):1214-1218
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study metabonomics in the urine of rats of different genders by magnetic resonance (MR) with 2 normalization methods. Methods Different normalization methods such as mean-centering not scaling (Ctr) and unit variance scaling (UV) were used before orthogonal to partial least squares discriminant analysis(OPLS-DA). Distinguished metabolites in the urine of different gender rats were analyzed by calculating the correlation coefficients. Results The data normalized by Ctr before OPLS-DA analysis revealed high degree conception metabolites in the urine such as valine, alanine, acetate, ornithine, aminohippurate, phenylethylamine, cytosine, citrate, dimethylamine, allantoin, methylamine, fumarate and one unknown metabolite whose chemical shift was δ4.14. Data normalized by UV before OPLS-DA analysis revealed the above 12 high degree conception metabolites except citrate, and also low degree conception metabolites such as thiamine, creatinine, formate and one unknown metabolite whose chemical shift was δ2.92.Conclusion Unit variance scaling is a more effective normalization method in metabonomic analysis.
