Effect of rheumatoid arthritis associated HLA-DRβ1 subtypes on protein kinase A signaling
- VernacularTitle:类风湿关节炎相关的HLA-DRβ1亚型对蛋白激酶A信号的影响
- Author:
Zhanguo LI
;
Liqun XIAO
;
Rulin JIA
;
Joseph HOLOSHITZ
- Publication Type:Journal Article
- From:
Chinese Journal of Internal Medicine
2001;40(1):19-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of rheumatoid arthritis (RA)-associated HLA-DRβ1*0401,*0402,*0403 and*0404 subtypes on protein kinase A (PKA) signaling. Methods To detect the activities of adenylate cyclase (AC), cAMP and PKA in transfectants expressing rheumatoid arthritis-associated HLA-DRβ1 subtypes and their mutants. Results The levels of AC, cAMP and PKA of HLA-DRβ1*0401 and*0404 transfectants were significant lower than that of HLA-DRβ1*0402 and*0403 transfectants (P＜0.01). The mutant HLA-DRβ1*0403 tranfectants expressing DRRAE or QRRAA secreted lower levels of PKA, cAMP and AC (P＜0.01). Conclusion RA-associated HLA-DRβ1*0401 and*0404 expression suppressed intracelluar PKA signaling pathway, and may mediate abnormal intracellular signaling in rheumatoid arthritis.
