Effects of prolactin on in-vitro interactions between thyrocytes from patients with Graves′ disease and autologous peripheral blood mononuclear cells
- VernacularTitle:催乳素对Graves 病甲状腺细胞与自体外周血单个核细胞体外相互作用的影响
- Author:
Jing LI
;
Zhongyan SHAN
;
Weiping TENG
- Publication Type:Journal Article
- From:
Chinese Journal of Internal Medicine
2001;40(2):82-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of prolactin on interactions between Graves′ disease (GD) thyrocytes and autologous peripheral blood mononuclear cells (PBMC) in coculture. Methods Primary thyrocytes and autologous PBMC were cocultured at various levels of ovine prolactin (oPRL) in vitro. The activation and proliferation of PBMC as well as HLA-DR and CD40 expressions on thyrocytes were determined by immunofluorescent staining and flow cytometry. Results With PBMC in GD cocultures, oPRL caused significant increase in the percentage of CD4+CD25+ cells at 200 μg/L ［(13.08±2.54)%, P＜0.01］ and in the proliferative index at 200 μg/L ［(17.82±3.02)%, P＜0.01］ and 1 000 μg/L ［(16.57±2.56)%, P＜0.05］, but not at 12.5 μg/L ［(10.43±2.38)% and (14.08±2.67)%, respectively］ or 50 μg/L ［(9.94±2.54)% and (14.56±2.80)%, respectively)］. The values at 200 or 1 000 μg/L as above were also significantly different from those at 12.5 or 50 μg/L. The percentage of CD40+ cells ［(48.25± 6.63)%，(52.28±6.94)%］ and delta mean fluorescence intensity (dMF; 42.94±10.24，49.51±12.34) were significantly lower in thyrocytes in GD cocultures treated with 200 or 1 000 μg/L oPRL than those untreated ［(58.38±6.62)% and 67.30±20.20］ or treated with two other concentrations of oPRL. The percentage of HLA-DR+ cells ［(46.79±7.51)%, P＜0.01］ and dMF (21.02±5.43, P＜0.01) were significantly higher in the thyrocytes treated with 50 μg/L oPRL than those untreated ［(33.51±8.58)% and 13.91±3.88］ or treated with three other concentrations. There were no significant differences between the dose groups of oPRL but those mentioned above. Conclusions Prolactin could affect interactions between GD PBMC and autologous thyrocytes and might eventually influence the shift of helper T cells. High prolactin levels might aggravate GD.
