Regulation of p53 and bcl-2 genes in apoptosis of human liver cancer cells induced by chemotherapy
- VernacularTitle:化疗诱导人肝癌细胞凋亡中p53和bcl-2基因的调控
- Author:
Jianqing YANG
;
Lianyue YANG
- Publication Type:Journal Article
- From:
Chinese Journal of Hepatobiliary Surgery
2001;7(1):31-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether the important apoptosis-related genes of p53 and bcl-2 participate in the regulation of apoptosis of human liver cancer cells by establishing the model of apoptosis of human liver cancer cells induced by mitomycin C (MMC). Methods The selected human liver cancer cell line HepG2 was treated with 8mg/ml MMC. The morphological changes of the cells were observed using fluorescent and electron transmission microscopy and the DNA fragments of the cells analyzed by agarose gel electrophoresis to testify cell apoptosis. The expression of p53 and bcl-2 genes was detected by flow cytometry. Results The morphological observation and biochemical examination showed that MMC induced the HepG2 cells to undergo apoptosis. After administration of MMC, the expression of p53 gene changed obviously while that of bcl-2 gene was not apparent. Conclusion p53 plays an important role in the regulation of apoptosis induced by MMC in HepG2 cells. This regulation may be an early event in apoptotic signal pathway. Meanwhile, the apoptosis of the cells is p53-dependent. However, bcl-2 gene may not participate in the control of this apoptosis.
