Fluorescence in situ hybridization detection of chromosome translocations induced by 60Co γ-rays in human lymphocytes dose-response curve and persistence
- VernacularTitle:用荧光原位杂交技术建立60Coγ射线诱发人外周血淋巴细胞染色体易位率剂量效应曲线及易位的持续性研究
- Author:
Xiaoling WANG
;
Jin LI
;
Zhiquan WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Radiological Medicine and Protection
2001;21(2):83-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study dose-response relationship for chromosome translocation and its persistence measured in human lymphocytes exposed to 60Co γ-rays.Methods The chromosome translocations in human peripheral lymphocytes were detected by fluorescence in situ hybridization using 4# and 7# combination of composite whole chromosome-specific DNA probes and Giemsa stain.Results The dose-response relationship for chromosome translocation induced by 60Co γ-rays in vitro could be described by the function:Y=0.0030+0.0134D+0.0165D2.The frequencies of chromosome translocations induced by 0.5 and 2 Gy γ-irradiation did not diminish over time,so it exhibited excellent persistence.Conclusions The results indicate that retrospective dose-reconstruction can be accomplished using chromosome translocation frequency.
