Effects of diagnostic ultrasound on expressions of P53 mRNA and Bcl-2 mRNA in spermatogenic cells in rats
- VernacularTitle:诊断超声辐照后大鼠睾丸生精细胞P53 mRNA、Bcl-2 mRNA表达改变
- Author:
Lianfang DU
;
Qingping ZHANG
;
Wangpeng LIU
- Publication Type:Journal Article
- From:
Chinese Journal of Ultrasonography
2001;10(3):182-184
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in the e xpressions of P53 mRNA and Bcl-2 mRNA in spermatogenic cells in rats after expo sure to ultrasound to elucidate the possible mechanisms underlying the apoptosis induced by ultrasound at genetic trascription level. Methods Thirty-two healthy male SD rats,（30±2） days old and (70±5) grams body weight, were randomized into 4 groups (n=8) according to the time of exposure to ultrasound,including control group without exposure,gr oups of 10 minutes, 20 minutes and 30 minutes with exposure to ultrasound for 10 ,20 and 30 min,respectively. Samples were taken 24 hours after exposure to ultra sound,then 4% paraformal-dehyde fixed,paraffin-embedded and sectioned into 4 μm sections (slides were pretreated with APES and poly-lysine). P53 and Bcl-2 in situ hybridization kits were purchased from Wuhan Boshida Bio-engineering C o.Results There was no significant difference in the expressions o f P53 mRNA or Bcl-2 mRNA in sperematogenic cells in rats between the control gr oup and 10 minutes group. The expressions of P53 mRNA in groups over 10 minutes were up-regulated and that of Bcl-2 mRNA were down-regulated. P53 mRNA in s permatogenic cells in rats was increased from (44.13±5.05)% in control group to (60.47±6.57)% in group 30 minutes. Bcl-2 mRNA was decreased f rom (40.29±9.10)% in control group to (31.01±5.67)% in group 3 0 minutes. Conclusions Exposure to ultrasound over 10 minutes can induce up -regulatory expressions of P53 mRNA and down-regulatory expressions of Bcl-2 mRNA in spermatogenic cells in rats.
