Inhibition of angiogenesis of human hepatocellular carcinoma by antisense oligodeoxynucleotide of vascular endothelial growth factor
- VernacularTitle:血管内皮生长因子反义寡核苷酸抑制肝癌的血管形成
- Author:
Hongguang WANG
;
Kaizong LI
;
Kefeng DOU
- Publication Type:Journal Article
- From:
Chinese Journal of General Surgery
2001;16(4):222-224
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether or not the antisense oligodeoxynucleotide (ODN) of vascular endothelial growth factor (VEGF) can supresses the protein expression of VEGF.Methods SMMC 7721 is a cell line that expresses VEGF.Therefore the medium conditioned by SMMC 7721 stimulates proliferation of bovine aortic endothelial cell (BAEC).Synthesized antisense ODN complementary to one region of VEGF mRNA was added into the medium of SMMC 7721.The expression of VEGF and the stimulation effect of SMMC 7721 was then determined.Result It was found that by means of inhibition of VEGF expression,the stimulation effect of SMMC 7721 was inhibited by the VEGF antisense ODN in a dose-dependent manner.At a dosage of 1,2.5,5,10μmol/L,the inhibition rate was 63.2%,82.4%,210% and 287.5%,respectively.Conclusion It is promising that VEGF antisense ODN works as a new gene therapeutic modaliey for antiangiogenesis of HCC.
