Research in QGY/CDDP hepatoma lines multi-drug resistance reversed by ultrasound contrast agent joint ultrasound mediating ASODN of drug resistance gene transfection
- VernacularTitle:超声造影剂联合超声介导耐药基因反义寡脱氧核苷酸转染肝癌细胞逆转多药耐药效应的研究
- Author:
Mingdong JIANG
;
Tingxiu XIANG
;
Shaolin LI
;
Yong YAN
;
Zhenghong WANG
;
Liang FANG
;
Xiaobo HUANG
;
Yu ZHAO
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Contrast media;
Carcinoma,hepatocellular;
Gene transfer techniques
- From:
Chinese Journal of Ultrasonography
2008;17(3):258-261
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the effect of QGY/CDDP hepatoma lines multi-drug resistance (MDR) reversed by gene transfection of mdr1,mrp-ASODN+ultrasound contrast agent+ultrasound.Methods The QGY/CDDP cells were transfected by mdr1,mrp ASODN+ultrasound contrast agent+ultrasound irradiation respectively and detected of the various indicators:cell adhesion rate,cell sensitivity to cell drug-resistance,the mRNA expression of mdr1 and mrp gene,the expression of P-gp and MRP protein. Results After mdr1-ASODN transfection,the drug sensitivity and expression of P-gp,MRP protein of QGY/CDDP cells were smaller changes(P＞0.05),and the rate of cells adherent and expression of resistance gene mRNA were obvious changes(P＜0.05).After mrp-ASODN transfection,the cells adherent rate,the drug sensitivity,the expression of resistance gene mRNA and P-gp,MRP protein were obvious changes respectively(P＜0.05),the experiment group(group 2')had bigger effects(P＜0.05).Conclusions mdr1. mrp-ASODN+ultrasound contrast agent+ultrasound irradiation could safely partly reverse MDR of hepatoma cells,which is a potential new approach for gene therapy.
