Effects of bioartificial kidney on cardiovascular function and cytokine response in endotoxic shock pigs
- VernacularTitle:生物人工肾对内毒素休克猪心血管功能的影响
- Author:
Jun XIA
;
Bangchang CHENG
;
Ming LEI
- Publication Type:Journal Article
- Keywords:
Bioartificial kidney;
Endotoxin shock;
Cardiovascular;
Cytokine;
Renal proximal tubule cells (PTCs)
- From:
Chinese Journal of Emergency Medicine
2005;14(7):535-540
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test the role of a bioartificial kidney which consisted of a continuous vein-vein hemofiltration (CVVH) system and renal proximal tubule cells (PTCs)device, also called the renal tubule assist device (RAD),in endotoxin shock.Methods Female crossbred pigs 25 to 30 kg received with 3×1011 bacteria / kg body weight of E.coli intraperitoneally followed by treatment with a sham RAD without cells (n=7) or a RAD with cells (n=7). Data on blood pressure, cardiac output, renal blood flow and systemic marker of inflammation (IL-6) were measured.Results Cardiac outputs and renal blood flows were higher in the RAD group with cells than in the sham RAD group after E.coli infusion (P＜0.05). Plasma IL-6 levels were lower in the cell RAD group than in the sham RAD group after bacterial administration (P＜0.05). A significant difference on survival time was also observed between the two groups,with(9.07 ± 0.88) h in the cell RAD group vs. (5.10 ± 0.46)h in the sham RAD group, P＜ 0.01.Conclusion The bioartificial kidney containing PTCs can improve cardiovascular function,reduce cytokine levels,and prolong survival time.
