Effect of calcitonin gene-related peptide on the expression of triggering receptor expressed on myeloid cells-1 in lipopolysaccharide-induced macrophages

VernacularTitle:降钙素基因相关肽对脂多糖诱导的巨噬细胞表达髓样细胞触发受体-1的影响
Author: Yunchao LI ; Chaxiang GUAN ; Yang ZHOU ; Guoying SUN ; Meng SHI ; Jing WU ; Wenjie LI
Publication Type:Journal Article
Keywords: calcitonin gene-related peptide; triggering receptor expressed on myeloid cells-1; lipopolysaccharide; macrophage
From: Journal of Central South University(Medical Sciences) 2010;35(2):100-106
CountryChina
Language:Chinese
Abstract: Objective To determine the effect of calcitonin gene-related peptide (CGRP) on triggering receptor expressed on myeloid cells-1 (TREM-1) in the lipopolysaccharide (LPS)-induced macrophages and its signal transduction pathway. Methods The levels of TREM-1 mRNA in the macrophages were observed by reverse transcription-polymerase chain reaction (RT-PCR), and flow cytometry was performed to detect TREM-1 protein expression levels in the macrophages. Results CGRP had no regulating effect on the expression of TREM-1 in the macrophages; LPS could up-regulate macrophages to express TREM-1; CGRP increased TREM-1 mRNA expression in LPS-induced macrophages in dose and time-dependent manner; CGRP increased TREM-1 protein expression in LPS-induced macrophages, which could be partially reversed by H-7 or H-89 (P<0.05). Conclusion CGRP can regulate the LPS-induced macrophages synthesis and secretion of TREM-1, and the intracellular signal transduction pathway is related to PKA and PKC.