Study on selective medium for isolation of Aspergillus fumigatus
- VernacularTitle:烟曲霉菌选择性培养基的实验研究
- Author:
Zehu LIU
;
Xulian Lü
;
Guixia Lü
;
Yongnian SHEN
;
Hongbin Xü
;
Wei CHEN
;
Suquan HU
;
Weida LIU
- Publication Type:Journal Article
- Keywords:
Aspergillus fumigatus;
Candida albicans;
Fluconazole;
Culture medium
- From:
Chinese Journal of Clinical Infectious Diseases
2008;1(1):34-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the growth inhibition of Aspergillns fumigatus by Candida albicans in vitro and to develop the selective medium for clinical isolation of Aspergillus fumigatus.Methods Aspergillus fumigatus and Candida albicans were single or co-cultured in sabouraud dextrose agar(SDA) medium and SDA broth in dark at 25 ℃,and fungal growth,pigmentation,as well as colony diameter weredocumented.Results ①The sensitivity of culture of Aspergillus fumigatus and Candida albicans on SDAplate was 100CFU/ml.②The growth of 106CFU/ml and 103CFU/ml Aspergills fumigatus was completely inhibited by 106CFU/ml Candida albicans.③Growth inhibition of Aspergillus fumigatus was correlated with the concentration of Candida albicans.④SDA containing 1 mg/L fluconazole inhibited growth of Candida albicans,and no Candida albicans was detected on SDA containing 5 mg/L and 25 mg/L fluconazole.Growth of Aspergillus fumigatus was partially inhibited on SDA containing 25 mg/L fluconazole.Conclusions Candida albicans can inhibit the growth of Aspergillus fumigatus in vitro.SDA containing 5 mg/L fluconazole can be used as the selective medium for the isolation of Aspergillus fumigatus.
