Scintillation Proximity Assay.
- Author:
Chang Woon CHOI
- Publication Type:Original Article
- Keywords:
Scintillation proximity assay;
Radioimmunoassay
- MeSH:
Allergy and Immunology;
Kinetics;
Ligands;
Microspheres;
Radioimmunoassay;
Radioisotopes;
Scintillation Counting
- From:Korean Journal of Nuclear Medicine
1999;33(6):461-465
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Scintillation proximity assay (SPA) is a unique type of radioimmunoassay and makes it possible to use radioisotopes for monitoring binding reactions continuously without separation procedure. Microbeads containing a fluorophor are covalently linked to antibody or receptor. When a radiolabeled antigen or ligand is added it binds to the beads and the emitted short range electrons, excite the fluorophor in the beads. The light emitted can be measured in a scintillation counter. 3H or 125I has been used for SPA. The sensitivities achieved with SPA are comparable to the sensitivities of other procedures. SPA is applicable to immunology, receptor binding, monitoring interactions of biomolecules and study for the kinetics of interaction between receptors and ligands.
