Orientia tsutsugamushi in unengorged chiggers of epidemiological area of tsutsugamushi by nested PCR technique
- Author:
Pham Xuan Da
- Publication Type:Journal Article
- Keywords:
tsutsugamushi
- MeSH:
Orientia tsutsugamushi;
Scrub Typhus;
Epidemiology;
Polymerase Chain Reaction
- From:Journal of Preventive Medicine
2005;15(1):47-51
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
The study applied the Nested PCR technique with part of 56kilodalton type-specific antigen (TSA) gene DNAs of Orientia tsutsugamushi. Forty-eight pools (480 individuals) of unengorged larvae belonging to 4 trombiculid species: L. scutellare, L. pallidum, L. kistasatoi and L. fuji, collected from soil and ground surface of an endemic area for tsutsugamushi disease, were tested for 56kilodalton type-specific antigen (TSA) gene DNAs of Orientia tsutsugamushi, by nested PCR. Among them, 2 of 13 pools of L. scutellare were positive, and 2 serotypes of Kuroki and Gilliam were identified. Remaining 35 pools of 3 species were negative for Orientia tsutsugamushi. By using epidemiological' analysis, unengorged larvae of L. scutellare was suspected as the vector of tsutsu-gamushi disease in the studied area.
