Clinicopathological significance of tumor necrosis factor-α and cytokeratin 20 expression in colonic cancer
- VernacularTitle:肿瘤坏死因子-α、细胞角蛋白20在结肠癌浸润中作用的研究
- Author:
Youpeng JIA
;
Zhongyu WANG
;
Li ZHANG
;
Yuanyuan WU
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
Tumor necrosis factor-alpha;
Keratins;
Reverse transcrip-tase polymerase chain reaction
- From:
Chinese Journal of Postgraduates of Medicine
2008;31(11):14-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression ofTNF- α mRNA and cytokeratin 20(CK20)mRNA in different tissue of colonic cancer patients, and the relations between the expression and the classify,invasion, as well as Dukes stage of colonic cancer. Methods RT-PCR method was used to detect the ex-pression of TNF-α mRNA and CK20 mRNA in 30 cases of colonic cancer, included cancer tissue,para-cancer tissue and normal tissue. Results The positive rate of TNF- α mRNA expressions in cancer tissue, para-cancer tissue and normal tissue were 70.0%, 43.3% and 20.0%, and the positive rate of CK20mRNA expressions were 63.3%, 33.3% and 16.7%, there were significant difference among the three tissues(P ＜ 0.01 ). But the expression of CK20 mRNA in para-cancer tissue had no significant difference compared with normal tissue (P＞ 0.05). The expression ofTNF- α mRNA was closely correlated with that of CK20mRNA.TNF- α mRNA and CK20 mRNA showed no significant difference in expressing of colonic cancer tissue (P ＞ 0.05 ), but TNF- α was closely correlated with Duke stage and depth of tumor invasion (P ＜ 0.05 ).Conclusion The expression of TNF- α mRNA is objective indicator associated with the invasion of the colonic cancer.
