Expression of aquaporin-8 in the hepatocytes of pregnant rats with intrahepatic cholestasis
- VernacularTitle:妊娠肝内胆汁淤积症孕鼠肝细胞中水通道蛋白8的表达
- Author:
Liqiong LI
;
Hongbo QI
- Publication Type:Journal Article
- Keywords:
Pregnancy complications;
Cholestasis,intrahepatic;
Aquaporins;
Rats
- From:
Chinese Journal of Obstetrics and Gynecology
2008;43(2):128-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the alteration on the expression levels of protein and messenger RNA(mRNA)of aquaporin-8(AQP8)in the hepatocytes of pregnant rats with intrahepatic cholestasis induced by ethinylestradiol.Methods A total number of 20 15-day pregnant rats were randomly divided into two groups:control group，intrahepatic cholestasis of pregnancy(ICP)group.In ICP group，rats were subcutaneously injected with ethinylestradiol(2.5 mg·kg-1·d-1)for 5 days to make the model of ICP.In control group，rats received subcutaneous injection of appropriate volume of propylene glycol for 5 days.The protein expression and subcellular localization of AQP8 in the hepatocytes of pregnant rats were detected using immunohistochemical methods.The mRNA expression of AQP8 in the hepatocytes was assayed by RT-PCR method.Results The model of ICP of pregnant ats was made successfully.Expressions of AQP8 protein and mRNA were detected in two groups.AQP8 protein level in ICP group was 10.8±2.4，significantly decreased than in control group 17.1±2.2(P＜0.05).AQP8 mRNA level in ICP group was 1.07±0.11，significantly higher than in control group 0.80±0.11(P＜0.05).Conclusion Down-regulated AQP8 protein expression and up-regulated AQP8 mRNA expression in the hepatocytes of pregnant rats with intrahepatic cholestasis may contribute to the pathogenesis of ICP.
