MRI assessment of acute myocardial infarction with transplantation of autologous mesenchymal stem cells in swine:an experimental study
- VernacularTitle:MRI评价骨髓问质干细胞治疗猪急性心肌梗死效果的实验研究
- Author:
Minjie LU
;
Shihua ZHAO
;
Haiyan QIAN
;
Shiliang JIANG
;
Yunqing WEI
;
Chaowu YAN
;
Yuejin YANG
;
Yuqing LIU
- Publication Type:Journal Article
- Keywords:
Myocardial infarction;
Mesenchymal stem cells transplantation;
Magnetic resonance imaging;
Animal experimentation
- From:
Chinese Journal of Radiology
2008;42(2):201-205
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of autologous bone marrow-derived mesenchymal stem cells (MSCs)transplantation on acute myocardial infarction in swine models using MRI. MethodsFourteen Chinese mini-pigs(27±3 kg)were divided into control group(n=7)and transplantation group(n=7).Acute myocardial infarction(AMI)model was made by occlusion of the left anterior descending coronary artery for 90 minutes,and then 10 ml autologous MSCs(3 × 106 cell/ml)were injected into LAD by over-wire-balloon catheter after one week. MRl was performed to assess the cardiac function and myocardial perfusion 1 week after AMI and 6 weeks after transplantation.The implanted cells in vitro were analyzed by immunofluorescence.ResuitsThe left ventricular ejection fraction(LVEF)in transplantation group was increased from(42.7 ±7.5)%to(50.1±10.1)%,which was significantly different from that in control group(P＜0.01).In addition,the dyskinetic segments in infarcted region and the infareted area were decreased by 4 and 3.2 cm2 respectively(P＜0.01),and the left ventricular weight index was increased by 4.1 g/m2 in transplantation group(P＜0.05)compared with control group.The DAPI-labeled cells in infarcted and peri-infarcted region indicated the survived MSCs.Immunofluoreseence also confirmed that those cells expressed cardiomyocyte-specific troponin T,connexin 43 and vessel-specific smooth muscle actin.Capillary density in both infarcted and peri-infarcted region were higher in transplantation group than the control group(P＜0.01).Conclusion MRI is a reliable imaging method for assessing the effects of stem cell transplantation in acute myocardial infartion of swine models.
