Apoptosis of neural cells in the neonatal rat cerebral cortex induced by 60Co Gamma Ray
- VernacularTitle:60Coγ射线诱导的新生大鼠大脑皮质神经细胞凋亡
- Author:
Shengli XU
;
Li SHEN
;
Biao CHEN
- Publication Type:Journal Article
- Keywords:
Ionizing radiation;
Cerebral cortex;
Apoptosis;
p53;
iNOS
- From:
Chinese Journal of Radiological Medicine and Protection
2008;28(2):99-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the cell death type and investigate the potential mechanism of ionizing radiation-induced neural cell death in the neonatal rat cerebral cortex.Methods The neonatal Wistar rats were given a single dose of 2.0 Gy γ-irradiation.The cell death type and characterization in cerebral cortex were identified using DNA electrophoresis,TUNEL and HE staining.The P53-and iNOS-positive cells were analyzed quantitatively using immunohistochemistry.Results The DNA and morphological characterization of death cells indicated that 2.0 Gy γ-irradiation induced apoptosis of the neural cells in neonatal rat cerebral cortex.The apoptosis indices in different cortex regions were significantly increased 4 h after irradiation,and reached the peak value at 12 h post-irradiation.The apoptosis index of neoconex was much higher than that of hippocampus(archicortex)and paleocortex,while paleocortex had lower apoptosis index than hippocampus.The quantitative immunohistoehemistry suggested that the numbers of P53 and iNOS-positive cells were not different between these three cortex regions at the same time-point after irradiation.Conclusion 2.0 Gy γ-rays induced apoptosis of the neural cells in neonatal rat cerebral codex.The response of cells to the damage effects of ionizing radiation was similar in different cortex regions;however,the apoptosis indices were different significantly.These findings imply that the developing phase or type of neural cells may play a pivotal role in the apoptosis process induced by ionizing radiation.
