The role of serum dihydropyrimidine dehydrogenase activity in patients with gastric cancer resistance to fluorouracil: a prospective study
- VernacularTitle:外周血二氢尿嘧啶脱氢酶活性对胃癌氟尿嘧啶化疗耐药影响的前瞻性研究
- Author:
Xiaohai ZHAO
;
Xuchao XUE
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Dihydropyrimidine dehydrogenase;
Fluorouracil;
Drug resistance,neoplasm;
Cell culture techniques
- From:
Chinese Journal of Postgraduates of Medicine
2008;31(12):6-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of the fluorouracil(FU)drug resistance and to prove deeply the role of serum dihydropyrimidine dehydrogenase(DPD)activity in patients with gastric cancer resistance to FU with the level of cell culture. Methods The peripheral blood was collected from 45 patients with advanced gastric cancer before chemotherapy to detect the activity of DPD by high performance liquid chromatography(HPLC).The patients were divided into two groups(high activity group and low activity group)according to the results. The FU drug inhibitory rate of gastric cancer cell cultured in vitro were tested with MTT to compare the effects of the two groups. Result The FU drug inhibitory rate of gastric cancer cell cultured in vitro was obviously lower in high activity group than that in low activity group,(47.6±±4.0)%,(53.7±8.0)%respectively. Conclusion The activity of serum DPD may be simple and convenient indicators in predicting the resistance to FU.
