Overexpression of HRAD17 sensitizes A549 cells to radiation-induced apoptosis
- VernacularTitle:HRAD17过表达促进辐射损伤诱导的A549细胞凋亡
- Author:
Yanhong GUO
;
Yun HAN
;
Yingbao ZHU
- Publication Type:Journal Article
- Keywords:
Cell apoptosis;
HRAD17;
p53 AIP1
- From:
Chinese Journal of Radiological Medicine and Protection
2008;28(2):132-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of overexpression of HRAD17 on radiation-induced apoptosis of A549 cells.Methods Transfection of FLAC-tagged HRAD17 expression plasmid into human A549 cells was carried out and flow cytometry was employed tO observe the apoptotic effect.In addition,phosphorylation of p53 Ser46 was detected by Westem blot and the transcription of p53 AIP1 was measured by RT-PCR.Results Overexpression of HRAD17 gene significantly increased radiation-induced apoptosis of A549 cells.Further.Ser46 phosphorylation of P53 protein was increased and transcription of p53 AIP1 gene was elevated in cells overexpressing HRAD17.Conclusions HRAD17 protein can sensitize A549 cells to radiation-induced apoptosis in part by increasing p53 AIP1 expression.
