The effects of cyclosporine A on immunoglobulins deposition in retina of streptozotocin-induced diabetic rats
- VernacularTitle:环孢素A对链脲菌素诱导的糖尿病大鼠视网膜免疫球蛋白异常沉积的影响
- Author:
Xin ZHANG
;
Jin CUI
;
Mingcai QIU
;
Deqiang LI
;
Peng ZHANG
;
Jinshi ZHANG
- Publication Type:Journal Article
- Keywords:
Diabetic retinopathy;
Immunoglobulins;
Cyelosporine
- From:
Chinese Journal of Internal Medicine
2008;47(2):125-128
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the autoimmune injuries on diabetic retinopathy(DR)and the protective effects of immunosuppre8sive therapy with eyelosporine A(CsA) on the DR of streptozotocin(STZ)-induced diabetic rats.Methods STZ-induced diabetic rats were randomly divided into 3 groups: group 1:diabetic group without any treatment;group 2:insulin-treated group;group 3:CsA-treated group which was further divided into 2 subgroups:subgroup A:CsA was given 1 week before hyperglycemia appeared and subgroup B:CsA was given one week after hyperglycemia appeared.Subgroup A and subgroup B were further subdivided into 3 groups respectively,based on the dose of CsA(1,4 and 8 mg·kg-1·d-1).As a control group,normal rats were also simultaneously monitored.The pathologic changes in the retina were investigated with HE stain and the deposition of immunoglobulins was detected with immunohistochemistry and immunofluorescent microscopy.Results After 8 week,the deposition of IgG, IgA and IgM was quite significant in the retina of diabetic rats.The data also suggested that insulin treatment had no effects on the DR.In contrast,with CsA intervention,the deposition of immunoglobulins on the retina of diabetic rats vanished.Conclusions Autoimmune injuries were shown,in the present study,to play a critical role in the pathogenesis of diabetic retinopathy.Immunosuppressive treatment with CsA showed protective effects by inhibiting the deposition of immunoglobulins in the retina of diabetic rats.
