Characterization of proteins in hyperlipidemia pancreas using differential gel electrophoresis and tandem mass spectrometry
- VernacularTitle:高脂血症对胰腺组织损伤机制的蛋白质组学分析
- Author:
Wei ZHANG
;
Xingpeng WANG
;
Zhuowei YU
;
Yue ZHU
;
Xiaofeng YU
;
Kai WU
;
Yue ZENG
;
Mingyi XU
- Publication Type:Journal Article
- Keywords:
Pancreas;
Hyperlipidemia;
Proteomics;
Differential in-gel electrophoresis
- From:
Chinese Journal of Pancreatology
2008;8(2):101-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of hypedipidemia on pancreas of rats by comparative proteomic analysis.Methods Ten male SD rats were randomly divided into two groups,the experimental group Was fed with high lipid forage and the control group Was fed with normal food.Pancreatic samples from the two groups were harvested six weeks later.Differential protein analysis Was performed using differential in-gel electrophoresis(DIGE),and characterizing the protein biomarkers using tandem mass spectrometry.Western blot Was used to confirm the expression of significantly changed proteins.Results Compared to the normal pancreas tissue,a total of 3 protein spot-features were found to be significantly increased and 11 significantly decreased in pancreatic samples with hyperlipidemia.Significantly increased proteins in hyperlipidemia pancreatic samples were arginaseⅡ,ribonuclease inhibitor and glyeine amidinotransferase,which increased by 2.19,1.82 and 1.12 fold,respectively.Significantly decreased proteins in hyperlipidemia group were tyrosyl-tRNA synthetase,alpha-amylase,triacylglycerol lipase,DJ-1protein,Cu,Zn superoxide dismutase,which dicreased by 2.48,2.37,1.85,1.73 and 1.65 folds,respectively.Western blot analysis revealed increased arginase Ⅱ levels and decreased alpha-amylase in pancreatic samples with hyperlipidemia.Conclusions Pancreas wag possibly injured by hyperlipidemia via increase of arginase Ⅱ.Decreased amylase and lipase may be the protection mechanism of pancreas.
