Ghrelin inhibit PAI-1 secretion induced by tumor necrosis factor-αvia p38MAPK in HepG2 cells
- VernacularTitle:P38MAPK介导Ghrelin对肿瘤坏死因子-α诱导的HepG2细胞纤溶酶原激活物抑制剂-1分泌的影响
- Author:
Liying DING
;
Hong ZHAO
;
Zhihong ZONG
;
Jian LI
;
Guoliang LIU
- Publication Type:Journal Article
- Keywords:
p38 mitogen-activated protein kinases;
Peptide hormones;
Tumor necrosis factor-alpha;
Hepatocytes;
Plasminogen activator inhihitor 1
- From:
Journal of Chinese Physician
2008;10(3):327-329
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ghrelin on PAI-1 secretion in HepG2 cells induced by TNF-αand the effect of p-38 MAPK.Methods HepG2 cells were cultured.The concentration of TNF-α used to treat the HepG2 cells wag selected.The effect of ghrelin on PAI-1 secretion induced by TNF-α was detected by ELISA,the p-38 MAPK expression was investigated by Western blot.Results The concentration of PAI-1 was increased when cells were exposed to different concentration of TNF-α.The p-p38 MAPK expression was increased when the cells were exposed to TNF-α,ghrelin could inhibit the increase of PAI-1 secretioN induced by TNF-α.The expression of p-p38 MAPK was decreased when the cells were pretreated with ghrelin.Conclusion PAI-1 secretion were increased after TNF-α in-creasing.Ghrelin could inhibit PAI-1 secretion via p38 MAPK.
