Inhibitory effect of antisense oligonucleotide of integrin-linked kinase on cell proliferation of human epithelial ovarian cancer
- VernacularTitle:整合素连接激酶反义寡核苷酸对卵巢上皮性癌细胞生长的抑制作用
- Author:
Qi LI
;
Yanhua ZHANG
;
Peiling LI
;
Junrui PEI
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Protein-serine-threonine kinases;
Oligonucleotides,antisense;
Cell line,tumor
- From:
Chinese Journal of Obstetrics and Gynecology
2008;43(3):218-222
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inhibitory effect of integrin-linked kinase antisense oligonucleotide(ILK-ASODN)on cell proliferation in human ovarian cancer cell line(HO8910).Methods We transfected ILK-ASODN into HO8910 to block ILK gene expression.measured the expression levels of integirn-linked kinase(ILK)mRNA by RT-PCR and ILK protein by western-blotting;the inhibiting effects of the transfection on HO8910 proliferation,the cell cycles,and cell apoptosis were assessed by water soluble tetrazolium-1(WST-1)and flow cytometry(FCM).Results After transfection of ILK-ASODN,the expression levels of ILK mRNA decreased significantly in groups D,E,F being 0.307±0.011,0.198±0.008,0,respectively,when compared with those of the two control groups of A and B(P＜0.05).The expression levels of ILK protein of the groups D,E and F decreased significantly also,being 26.3 ± 0.8,20.6±0.4 and O,respectively.HO8910 cell proliferation was inhibited significantly,and the rates of apoptosis of the groups D,E and F increased significantly,being 7.31%,8.84%and 11.27%respectively.The cell population increased in G0/G1 phase of the groups D,E and F,being 49.25%,56.28%and 67.61%respectively.significantly different in comparison with those of groups of A and B (P＜0.01).Conclusions Transfection of ILK-ASODN into human ovarian cancer line inhibited cancer cell proliferation significantly.
