β1,β2 and β3-adrenoreceptor mRNA expression in the rat sepsis model
- VernacularTitle:脓毒症大鼠心肌β1、β2、β3肾上腺素能受体mRNA表达水平的研究
- Author:
Bo ZHEN
;
Chunfeng LIU
- Publication Type:Journal Article
- Keywords:
β-adrenoreceptors;
Sepsis;
Rats
- From:
Chinese Pediatric Emergency Medicine
2008;15(2):134-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate β1、β2 and β3-adrenoreceptor mRNA expression in the sepsis rat model and to investigate the influence of the levels of β1、β2(especially β3AR)on heart dysfunction of sepsis rats.Methods The rats were randomly divided into two groups:control group and sepsis group,either group was then divided into four subgroups:6 h,18 h,24 h,36 h,RT-PCR was used to determined β1、β2 and β3-adrenoreceptor mRNA expression.Results The ratio of β1-AR/β-actin mRNA was lower in sepsis groups as compared with control.Among the two groups,the control group had the highest ratio(1.07±0.13)and sepsis 36h group had the lowest(0.59±0.13)with significant difference(P＜0.05).The ratio of β2-AR/β-actin mRNA was lower in sepsis group as mmpared with coatrol,but there was no statistical significance.The ratio of β3-AR/β-actin mRNA was higher in sepsis group as compared with control.Sepsis 36 h group was the highest with an increase of 73%as compared with control(P＜0.05).Conclusion These data suggest that the reduction of β1-AR mRNA and the increase of β3-AR mRNA might contribute to sepsis-related cardiac dysfunction.
