Identification of cDNAs of Genes Suppressed during the Process of Atrophy in Rat Skeletal Muscle.
- Author:
Hee Chul MOON
1
;
Myung Soo YOUN
;
Sang Jin CHEON
;
Hui Taek KIM
;
Chul Min KIM
;
Jeung Tak SUH
Author Information
1. Department of Orthopedic Surgery, Pusan National University School of Medicine, Pusan, Korea. jtsuh@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Rat;
Skeletal muscle;
Atrophy;
Genes;
Expression
- MeSH:
Animals;
Atrophy;
Biotin;
Calcium;
Carbohydrate Metabolism;
Chimera;
Clinical Coding;
Contractile Proteins;
DNA;
DNA, Complementary;
Down-Regulation;
Femoral Nerve;
Gene Expression;
Gene Expression Profiling;
Gene Library;
Glycogenolysis;
Glycolysis;
Hypertrophy;
Leg;
Muscle, Skeletal;
Muscles;
Muscular Atrophy;
Oxidative Phosphorylation;
Proteins;
Rats;
Sciatic Nerve;
Up-Regulation
- From:Journal of Korean Orthopaedic Research Society
2008;11(1):18-30
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To elucidate the molecular basis of muscle atrophy in cellular adaptation point of view, gene expression profiling in rat muscle atrophy model was performed. The functions changed by muscle atrophy were analyzed. MATERIAL AND METHODS: Sciatic nerve and femoral nerve were resected in right leg to make muscle atrophy model in rat. The left leg was considered as a compensatory hypertrophy model. The suppression subtractive hybridization (SSH) was done to identify the profile of differential gene expression during muscle atrophy followed by nerve injury in rat. The DNA fragments obtained in SSH were labeled with biotin and used as cDNA tags for isolation of full-length cDNA from cDNA library. Differentially expressed genes were confirmed by reverse dot blot hybridization. RESULTS: Down regulation of genes were much more predominant than up regulation. The profile of down regulated genes were composed of genes coding muscle contractile proteins, enzymes involving carbohydrate metabolism including glycolysis and glycogenolysis, enzymes in oxidative phoshorylation, and proteins related with calcium release. The target genes were isolated by enrichment using cDNA tags from cDNA library for further functional studies. We identified some novel genes related with muscle atrophy by nerve injury. CONCLUSION: During the process of muscle atrophy, genes coding muscle contractile proteins, enzymes in carbohydrate metabolism, enzymes in oxidative phosphorylation, and proteins related with calcium release were down regulated.
