The expression of GSTπ in the HepG2 cell after multiple thermotherapy
- VernacularTitle:重复加热对肝癌HepG2细胞谷胱苷肽-S-转移酶π表达的影响
- Author:
Chunhong QIN
;
Yongguo LI
;
Jang WU
;
Kuhan HE
;
Xing SHEN
;
Shuke FEI
- Publication Type:Journal Article
- Keywords:
Hyperthermia,induced;
Liver neoplasms;
Glutathione transferase
- From:
Journal of Chinese Physician
2008;10(3):297-299
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression change of ghtathione S-transferase π(GSTπ)protein and mRNA in the HepG2 cell after multiple thermotherapy.Methods HepG2 cells were treaded by ten repeated cycles of exposure at 43 degree C for 80 minutes twice a day，the sensibility of HepG2 cell to Adriamycin was analyzed by MTr assay，and the expression of QSTπprotein and mRNA were detected by immunohistochemical method and RT-PCR.Results The drug resistance to Adriamycin was gained by HepG2 cells after multiple thermotherapy，and the expression of GSTπ protein and mRNA wag strengthened.Conclusions The resistance of heated HepG2 cells to chemotherapeutics was concerned with overexpression of GSTπ protein and mRNA.
