Effect of phorbol-12-myristate-13-acetate on cyclooxygenase-2 expression in human HaCaT keratinocytes
- VernacularTitle:佛波酯对HaCaT细胞环氧合酶-2表达水平的影响
- Author:
Guiying ZHANG
;
Xiaoyun YANG
;
Mingliang CHEN
;
Shengbo YANG
;
Shenghua LI
;
Qianjin LU
;
Yuwen SU
;
Rong XIAO
;
Haiquan WEN
- Publication Type:Journal Article
- Keywords:
Cyclooxygenase 2;
Phorbol-12-myristate-13-acetate;
HaCaT cells
- From:
Chinese Journal of Dermatology
2008;41(4):241-243
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of phorbol-12-myristate-13-acetate(PMA)on cyclooxygenase-2(COX-2) mRNA and protein expression in cultured human HaCaT keratinocytes,and the mechanism for cytotoxity of PMA against keratinocytes.MethodsRT-PCR and Westem blot were utilized to detect the expression of COX-2 mRNA and protein in cultured HaCaT ells at 24 hours after the treatment with various concentrations of PMA (0.1,1.0,10 mg/L).ResultsWithout any treatment,there was no or a weak expression df COX-2 mRNA and protein in HaCaT cells;incubation witll PMA resulted in the induction of the expression of COX-2 in HaCaT cells.The expression levels of COX-2 mRNA and protein in 10 mg/L PMA-pretreated HaCaT cells were significantly higher than those in 1.0 mg/L PMA-pretreated HaCaT cells,which was in turn higher than that in 0.1 mg/L PMA-pretreated cells and untreated cells;the difrerence was statistically significant (all P＜0.01).Conclusion These results suggest that PMA may be involved in keratinocyte tumorigenesis by upregulating he expression of COX-2 as well as synthesis and release of prostaglandin in keratinocytes.
