Reverse dot blot compared with hybrid capture 2 to detect human papillomavirus infection in cervix
- VernacularTitle:反向斑点杂交技术和杂交捕获法检测子宫颈人乳头瘤病毒感染的对比
- Author:
Haiya WU
;
Zheng ZHU
;
Shaojuan WANG
- Publication Type:Journal Article
- Keywords:
Papillomavirus infections;
Uterine cervical neoplasms;
Two-Hybrid system techniques
- From:
Chinese Journal of Primary Medicine and Pharmacy
2008;15(3):359-360
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the capacity of reverse dot blot(RDB)technique in screening Human papillomavirus infection in cervix.Methods 49 cases examined by hybrid capture 2(HC2)were collected for RDB technique testing.Results In the 49 cases,the positive of HR-HPV examined by HC2 was 89.8%(44/49),53.1%(26/49)by RDB,respectively.Sensitivity,specificity of RDB technique was 59.1%,100(5/5),conordance rate was 63.3%(31/49),positive predictive value and negative predictive value of RDB was 100%,21.7%,respectivdy.Kappa=0.23.Conclusion It is superior in the economy for screening human papillomavirus infection in cervix that RDB technique is developed and optimized,cheap RDB technique should be substituted for expensive HC2.
