17β-estradiol modulates bone-marrow derived dendritic cells in(NZB×NZW)F1 female mice
- VernacularTitle:雌二醇调控新西兰黑鼠×白鼠子一代雌鼠的骨髓树突状细胞作用研究
- Author:
Bo JIANG
;
Lingyun SUN
;
Hong WANG
- Publication Type:Journal Article
- Keywords:
Estradiol;
Dendritic cells;
Bone marrow;
(NZBxNZW)F1 mice
- From:
Chinese Journal of Rheumatology
2008;12(4):234-237
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanisms of estrogen in the pathogenesis of systemic lupus erythematosus(SLE),we compared the effects of 17β-estradiol(E2)on bone marrow(BM)-derived dendritic cells (BMDCs)in(NZBxNZW)F1(NZB/w F1)female mice befbre and after the disease onset.Methods Immature,BMDCs were differentiated from BM monocytes cultured with rmGM-CSF,rmlL-4,E2 and estrogen receptor (ER)modulator-tamoxifen(TAM)for 7 days.Then immature DCs were stimulated by LPS for 24h to get mature BMDCs.Flow cytometry was utilized to detect the production of costimulatory molecule CD40 and intracellular IL-6,IL-10,IL-12 and TNFα of DCs.Mixed lymphocytes reaction was used to measure the stim-ulatory activity of DCs on spleen T lymphocytes.Results E2 mainly increased the expression of CD40 on im-mature BMDCs but reduced its expression on mature BMDCs.E2 enhanced the stimulatory activity of immature BMDCs,but weakened the activity of mature BMDCs.E2 decreased the production of cytokines of BMDCs in young mice,but increased them in old mice.Conclusion E2 modulates the functions of BMDCs of lupus model mice by binding with ER.The modulation varies depending on disease progression and cell maturation status.
