Effect of RNAi-mediated STAT3 gene silencing on the invasiveness of human pancreatic cancer cells
- VernacularTitle:RNAi沉默STAT3对人胰腺癌细胞侵袭能力的影响
- Author:
Chen HUANG
;
Zhengjun QIU
;
Tao JIANG
;
Lin ZHU
;
Fang ZHANG
;
Kejian HUANG
;
Jun CAO
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
RNA interference;
Signal transduction and transcription;
Invasion;
Metastasis
- From:
Chinese Journal of General Surgery
2008;23(4):292-295
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of RNAi-mediated sTAT3 gene silencing on the invasiveness of human pancreatic cancer cells. Methods STAT3 shRNA expression vector was stably transfected to SW1990 cells.STAT3 mRNA and protein expression were examined using reverse transcription polymerase chain reaction(RT-PCR)and Western blot,respectively.The invasion ability of SW1990 cells was determined by cell invasion assay in vitro.RT-PCR and Westem blot were performed to detect the mRNA and protein expression of the MMP-2 and VEGF,respectively. Results mRNA and protein expression of STAT3 were inhibited significantly by stable transfection of STAT3 shRNA expressing vectors.STAT3 silence with RNAi significantly inhibited the invasion ability of SW1990 cells decreasing protein and mRNA expression of MMP-2 and VEGF in SWl990 cells. Conciusion STAT3 silence with RNAi significantly inhibits the invasion ability of pancreatic cancer cells through down-regulating MMP-2 and VEGF.
